Helicobacter pylori transiently in the mouth may participate in the transmission of infection

Memórias do Instituto Oswaldo Cruz

Denise G Silva²  Eduardo Mb Tinoco²  Gifone A Rocha¹  Andreia Maria Camargos Rocha¹  Juliana B Guerra¹  Ivan Eb Saraiva¹  Dulciene Mm Queiroz¹ 

[1] ,Universidade do Grande Rio Escola de Odontologia Duque de Caxias RJ ,Brasil

关键词: H. pylori;    dental plaque;    saliva;    oral cavity;    transmission;    vacA;   

DOI  :  10.1590/S0074-02762010000500009

来源: SciELO

【 摘 要 】

Helicobacter pylori infection is associated with peptic ulcer and gastric carcinoma. The oral cavity may be a reservoir for H. pylori; however, the results of studies on this subject are controversial. We employed single-step and nested polymerase chain reactions (PCR) to detect the presence of the vacA, ureA and 16S rDNA genes of H. pylori in the stomach, saliva and dental plaque of 30 subjects. The results were confirmed by sequencing. Nested 16S rDNA and ureA amplification was achieved in 80% of gastric, 30% of saliva and 20% of dental plaque specimens. Sequencing of 10, seven and four 16S rDNA products from stomach, saliva and dental plaque, respectively, showed > 99% identity with H. pylori. Sequencing of the other four oral cavity PCR products showed similarity with Campylobacter and Wolinella species. Additionally, the vacA genotype identified in the samples of different sites was the same within a given subject.H. pylori may be found in the oral cavity of patients with gastric infection, thus it could be a source of transmission. However, results obtained with detection methods based only on PCR should be interpreted with caution because other microorganisms that are phylogenetically very close to H. pylori are also present in the mouth.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License