Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Memórias do Instituto Oswaldo Cruz

Raquel Elvira Ocazionez²  Rocio Meneses²  Flor Ángela Torres²  Elena Stashenko¹ 

[1],Centro de Investigaciones en Enfermedades Tropicales Laboratorio de Arbovirus

关键词: essential oil;    antiviral;    virucidal activity;    dengue virus;    Lippia sp;   

DOI  :  10.1590/S0074-02762010000300010

来源: SciELO

【 摘 要 】

The inhibitory effect of Lippia alba and Lippia citriodora essential oils on dengue virus serotypes replication in vitro was investigated. The cytotoxicity (CC50) was evaluated by the MTT assay and the mode of viral inhibitory effect was investigated with a plaque reduction assay. The virus was treated with the essential oil for 2 h at 37ºC before cell adsorption and experiments were conducted to evaluate inhibition of untreated-virus replication in the presence of oil. Antiviral activity was defined as the concentration of essential oil that caused 50% reduction of the virus plaque number (IC50). L. alba oil resulted in less cytotoxicity than L. citriodora oil (CC50: 139.5 vs. 57.6 μg/mL). Virus plaque reduction for all four dengue serotypes was observed by treatment of the virus before adsorption on cell. The IC50 values for L. alba oil were between 0.4-32.6 μg/mL and between 1.9-33.7 μg/mL for L. citriodora oil. No viral inhibitory effect was observed by addition of the essential oil after virus adsorption. The inhibitory effect of the essential oil seems to cause direct virus inactivation before adsorption on host cell.

【 授权许可】

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