Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis

Memórias do Instituto Oswaldo Cruz

João Batista Júnior²  Rodrigo Berzaghi¹  Adelita Duanra Medeiros Do Monte Arnaud²  Cor Jesus Fernandes Fontes²  Zoilo Pires De Camargo¹  Rosane Christine Hahn² 

[1] ,Universidade Federal de Mato Grosso Faculdade de Ciências Médicas Laboratório de MicologiaCuiabá MT ,Brasil

关键词: Paracoccidioides brasiliensis;    simultaneous infection;    RAPD;   

DOI  :  10.1590/S0074-02762010000100009

来源: SciELO

【 摘 要 】

This study is the first report on genetic differences between isolates of Paracoccidioides brasiliensis from a single patient. We describe a simultaneous infection with genetically distinct isolates of P. brasiliensis in a patient with chronic paracoccidioidomycosis. The clinical isolates were obtained from lesions in different anatomical sites and were characterised by random amplified polymorphic DNA (RAPD) analysis. The RAPD technique can be helpful for distinguishing between clinical isolates. Different random primers were used to characterise these clinical isolates. The RAPD patterns allowed for differentiation between isolates and the construction of a phenetic tree, which showed more than 28% genetic variability in this fungal species, opening new possibilities for clinical studies of P. brasiliensis. Based on these results and preliminary clinical findings, we suggest that different genotypes of P. brasiliensis might infect the same patient, inducing the active form of the disease.

【 授权许可】

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