Analysis of the genetic polymorphism of Paracoccidioides brasiliensis and Paracoccidioides cerebriformis "Moore" by random amplified polymorphic DNA (RAPD) and 28S ribosomal DNA sequencing: Paracoccidioides cerebriformis revisited

Revista do Instituto de Medicina Tropical de São Paulo

Sarah Desirée Barbosa Cavalcanti²  José Eduardo Levi¹  Kátia Cristina Dantas²  José Eduardo Costa Martins² 

[1] ,Instituto de Medicina Tropical de São Paulo Laboratório de Micologia Médica

关键词: Paracoccidioides cerebriformis;    Paracoccidioides brasiliensis;    Aspergillus penicillioides;    RAPD;    28S rDNA sequence;   

DOI  :  10.1590/S0036-46652005000300001

来源: SciELO

【 摘 要 】

Our purpose was to compare the genetic polymorphism of six samples of P. brasiliensis (113, 339, BAT, T1F1, T3B6, T5LN1), with four samples of P. cerebriformis (735, 741, 750, 361) from the Mycological Laboratory of the Instituto de Medicina Tropical de São Paulo, using Random Amplified Polymorphic DNA Analysis (RAPD). RAPD profiles clearly segregated P. brasiliensis and P. cerebriformis isolates. However, the variation on band patterns among P. cerebriformis isolates was high. Sequencing of the 28S rDNA gene showed nucleotide conservancy among P. cerebriformis isolates, providing basis for taxonomical grouping, and disclosing high divergence to P. brasiliensis supporting that they are in fact two distinct species. Moreover, DNA sequence suggests that P. cerebriformis belongs in fact to the Aspergillus genus.

【 授权许可】

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