In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis

Memórias do Instituto Oswaldo Cruz

Mirela Verza²  Raquel De Abreu Maschmann²  Márcia Susana Nunes Silva²  Elis Regina Dalla Costa²  Marta Osório Ribeiro²  Franciele Rosso²  Philip Noel Suffys¹  Enrico Tortoli¹  Fiorella Marcelli¹  Arnaldo Zaha¹  Maria Lucia Rosa Rossetti² 

[1] ,Fundação Estadual de Produção e Pesquisa em Saúde Centro de Desenvolvimento Científico e Tecnológico Porto Alegre RS ,Brasil

关键词: drug resistance;    isoniazid;    katG315;    reverse hybridization assay;    tuberculosis;   

DOI  :  10.1590/S0074-02762009000500008

来源: SciELO

【 摘 要 】

Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC→ACC (Ser→Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and, possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315 mutation in 100% of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.

【 授权许可】

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