Delay in maturation of the submandibular gland in Chagas disease correlates with lower DNA synthesis

Memórias do Instituto Oswaldo Cruz

José B Alves¹  Marcelo H Napimoga¹  Marcelo S Araujo¹  Gerluza Ab Silva² 

[1] ,Universidade de Uberaba Laboratório de Biologia Molecular Uberaba MG ,Brasil

关键词: submandibular gland;    Trypanosoma cruzi;    Chagas disease;    immunohistochemistry;   

DOI  :  10.1590/S0074-02762008000600012

来源: SciELO

【 摘 要 】

It has been demonstrated that the acute phase of Trypanosoma cruzi infection promotes several changes in the oral glands. The present study examined whether T. cruzi modulates the expression of host cell apoptotic or mitotic pathway genes. Rats were infected with T. cruzi then sacrificed after 18, 32, 64 or 97 days, after which the submandibular glands were analyzed by immunohistochemistry. Immunohistochemical analyses using an anti-bromodeoxyuridine antibody showed that, during acute T. cruzi infection, DNA synthesizing cells in rat submandibular glands were lower than in non-infected animals (p < 0.05). However, after 64 days of infection (chronic phase), the number of immunolabeled cells are similar in both groups. However, immunohistochemical analysis of Fas and Bcl-2 expression did not find any difference between infected and non-infected animals in both the acute and chronic stages. These findings suggest that the delay in ductal maturation observed at the acute phase of Chagas disease is correlated with lower expression of DNA synthesis genes, but not apoptotic genes.

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