A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni

Memórias do Instituto Oswaldo Cruz

Coen M Adema²  Mei-zhong Luo¹  Ben Hanelt²  Lynn A Hertel¹  Jennifer J Marshall²  Si-ming Zhang²  Randall J Dejong²  Hye-ran Kim¹  David Kudrna¹  Rod A Wing¹  Cari Soderlund¹  Matty Knight¹  Fred A Lewis¹  Roberta Lima Caldeira¹  Liana K Jannotti-passos¹  Omar Dos Santos Carvalho¹  Eric S Loker² 

[1] ,University of New Mexico Department of Biology Albuquerque NM ,US

关键词: genomics;    gene discovery;    fingerprinting;    schistosomiasis;    medical malacology;   

DOI  :  10.1590/S0074-02762006000900027

来源: SciELO

【 摘 要 】

To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (biology.unm.edu/biomphalaria-genome.html), working with the Arizona Genomics Institute (AGI) and supported by the National Human Genome Research Institute (NHGRI), produced a high quality bacterial artificial chromosome (BAC) library. The BB02 strain B. glabrata, a field isolate (Belo Horizonte, Minas Gerais, Brasil) that is susceptible to several strains of Schistosoma mansoni, was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBAC1 vector. The resulting B. glabrata BAC library (BG_BBa) consists of 61824 clones (136.3 kb average insert size) and provides 9.05 × coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clones indicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads, 299860 nt) indicated that the genome of B. glabrata contains ~ 63% AT, and disclosed several novel genes, transposable elements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI at cost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI.

【 授权许可】

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