Genetics and Molecular Biology | |
Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids | |
Jillian M. Hagel2  Raz Krizevski1  Korey Kilpatrick1  Yaron Sitrit1  Frédéric Marsolais1  Efraim Lewinsohn1  Peter J. Facchini2  | |
[1] ,University of Calgary Department of Biological Sciences Alberta,Canada | |
关键词: khat; (S)-cathinone; phenylpropylamino alkaloids biosynthesis; EST library; gene discovery; | |
DOI : 10.1590/S1415-47572011000400017 | |
来源: SciELO | |
【 摘 要 】
Khat (Catha edulis Forsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenylpropylamino alkaloids (1S,2S)-cathine and (1R,2S)-norephedrine is not well characterized in plants. We prepared a cDNA library from young khat leaves and sequenced 4,896 random clones, generating an expressed sequence tag (EST) library of 3,293 unigenes. Putative functions were assigned to > 98% of the ESTs, providing a key resource for gene discovery. Candidates potentially involved at various stages of phenylpropylamino alkaloid biosynthesis from L-phenylalanine to (1S,2S)-cathine were identified.
【 授权许可】
CC BY
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