Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil

Memórias do Instituto Oswaldo Cruz

Ana Maria Tavares Borges²  José Marcus Sócrates Teixeira¹  Paulo Sérgio Sucasas Da Costa¹  Loreny Gimenes Giugliano¹  Fabíola Souza Fiaccadori²  Rachel De Carvalho E Franco²  Wilia Marta Elsner Diederichsen De Brito²  José Paulo Gagliardi Leite¹  Divina Das Dores De Paula Cardoso² 

[1] ,Instituto de Patologia Tropical e Saúde Pública Laboratório de Virologia

关键词: calicivirus;    acute gastroenteritis;    reverse transcriptase;    polymerase chain reaction;    Brazil;   

DOI  :  10.1590/S0074-02762006000700003

来源: SciELO

【 摘 要 】

The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period.

【 授权许可】

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