Different response to inflammation of the multiple mRNAs of rat N‐acetylglucosaminyltransferase I with variable 5′‐untranslated sequences

FEBS Letters

Kioka, Noriyuki¹  Sakata, Shohei¹  Yamada, Masayasu²  Nishiu, Jun¹  Komano, Tohru¹  Fukada, Takashi¹ 

[1] Laboratory of Biochemistry, Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan;Laboratory of Animal Reproduction, Division of Animal Science, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan

关键词: Glycosyltransferase;    Inflammation;    Transcriptional regulation;    N-Acetylglucosaminyltransferase I;    GnT-I;    N-acetylglucosaminyltransferase I;    IL;    interleukin;    TNF;    tumor necrosis factor;    UTR;    untranslated region;    RACE;    rapid amplification of cDNA ends;    PCR;    polymerase chain reaction;    RT;    reverse transcriptase;    MMLV;    Moloney murine leukemia virus;    ORF;    open reading frame;   

DOI  :  10.1016/S0014-5793(98)01136-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We found that there are at least five subclasses of N-acetylglucosaminyltransferase I (GnT-I; EC 2.4.1.101) mRNA with different 5′-untranslated regions in rat brain. These five subclasses were also expressed in many tissues with distinct tissue-specific patterns. Moreover, they were regulated differently in response to acute-phase inflammation. The expression of the most abundant subclass of GnT-I mRNA in rat liver decreased 2.5-fold in response to inflammation, concomitantly with a significant decrease in the total amount of GnT-I mRNA. In contrast, one of the minor subclasses of GnT-I mRNA was induced 10-fold by inflammation.

【 授权许可】

Unknown