Identification of Anopheles (Nyssorhynchus) albitarsis complex species (Diptera: Culicidae) using rDNA internal transcribed spacer 2-based polymerase chain reaction primes

Memórias do Instituto Oswaldo Cruz

Cong Li¹  Richard C Wilkerson¹ 

[1] ,Walter Reed Army Institute of Research Dept. of Entomology Silver Spring MD ,US

关键词: Anopheles albitarsis;    species complex;    polymerase chain reaction identification;    malaria;    internal transcribed spacer 2;   

DOI  :  10.1590/S0074-02762005000500009

来源: SciELO

【 摘 要 】

Anopheles (Nyssorhynchus) marajoara is a proven primary vector of malaria parasites in Northeast Brazil, and An. deaneorum is a suspected vector in Western Brazil. Both are members of the morphologically similar Albitarsis Complex, which also includes An. albitarsis and an undescribed species, An. albitarsis "B". These four species were recognized and can be identified using random amplified polymorphic DNA (RAPD) markers, but various other methodologies also point to multiple species under the name An. albitarsis. We describe here a technique for identification of these species employing polymerase chain reaction (PCR) primers based on ribosomal DNA internal transcribed spacer 2 (rDNA ITS2) sequence. Since this method is based on known sequence it is simpler than the sometimes problematical RAPD-PCR. Primers were tested on samples previously identified using RAPD markers with complete correlation.

【 授权许可】

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