Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil

Memórias do Instituto Oswaldo Cruz

Regina Ms Amorim²  Claudiner P Oliveira²  Patrícia S Wyant²  Daniela M Cerqueira²  Geni Nl Câmara¹  Luciano S Flores¹  Regina Mb Martins¹  Cláudia Rf Martins² 

[1] ,Universidade de Brasília Instituto de Biologia Departamento de Biologia CelularBrasília DF ,Brasil

关键词: hepatitis C virus;    genotypes;    Federal District;    Central Brazil;   

DOI  :  10.1590/S0074-02762004000800019

来源: SciELO

【 摘 要 】

The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3.

【 授权许可】

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