Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR

Memórias do Instituto Oswaldo Cruz

Kelly Grace Magalhães¹  Liana Konovaloff Jannotti Passos¹  Omar Dos Santos Carvalho¹ 

[1] ,Centro de Pesquisas René Rachou-FiocruzBelo Horizonte MG ,Brasil

关键词: Fasciola hepatica;    Lymnaea columella;    multiplex polymerase chain reaction;    DNA mitochondrial;    diagnosis;   

DOI  :  10.1590/S0074-02762004000400013

来源: SciELO

【 摘 要 】

From complete mitochondrial DNA sequence of Fasciola hepatica available in Genbank, specific primers were designed for a conserved and repetitive region of this trematode. A pair of primers was used for diagnosis of infected Lymnaea columella by F. hepatica during the pre-patent period simultaneously with another pair of primers which amplified the internal transcribed spacer (ITS) region of rDNA from L. columella in a single Multiplex-PCR. The amplification generated a ladder band profile specific for F. hepatica. This profile was observed in positive molluscs at different times of infection, including adult worms from the trematode. The Multiplex-PCR technique showed to be a fast and safe tool for fascioliasis diagnosis, enabling the detection of F. hepatica miracidia in L. columella during the pre-patent period and identification of transmission areas.

【 授权许可】

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