期刊论文详细信息
Memórias do Instituto Oswaldo Cruz
Aspects of the Maintenance of the Life Cycle of Fasciola hepatica in Lymnaea columella in Minas Gerais, Brazil
Cecília Pereira De Souza2  Kelly Grace Magalhães2  Liana Konovaloff Jannotti Passos2  Glaucia Cristina Pereira Dos Santos2  Fabio Ribeiro1  Naftale Katz1 
[1] ,Fiocruz Centro de Pesquisas René Rachou-Fiocruz Laboratório de Malacologia
关键词: fascioliasis;    Lymnaea columella;    infection;    Fasciola hepatica;    Minas Gerais;    Brazil;   
DOI  :  10.1590/S0074-02762002000300023
来源: SciELO
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【 摘 要 】

Fascioliasis is a parasitic disease of domestic ruminants that occurs worldwide. The lymnaeid intermediate hosts of Fasciola hepatica include Lymnaea columella, which is widely distributed in Brazil. A colony of L. columella from Belo Horizonte, MG, was reared in our laboratory to be used in studies of the F. hepatica life cycle, the intermediate host-parasite relationship and development of an anti-helminthic vaccine. In the first experiment 1,180 snails were exposed to miracidia of F. hepatica eggs removed from the biliary tracts of cattle from the State of Rio Grande do Sul. In the second and third experiments the snails were exposed to miracidia that had emerged from F. hepatica eggs from Uruguay, maintained in rabbits. The rates of infection in the first, second and third experiments were 0, 42.1 and 0% respectively. Over 15,806 metacercariae were obtained and stored at 4ºC. Four rabbits weighing 1.5 kg each were infected with 32-44 metacercariae and two with 200. Three rabbits begin to eliminate eggs of the parasite in the feces from 84 days after infection onwards. The biological cycle of F. hepatica in L. columella and the rabbit was completed within 124 days.

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