An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts

Memórias do Instituto Oswaldo Cruz

Erick Vaz Guimarães²  Laís De Carvalho¹  Helene Santos Barbosa² 

[1] ,Fiocruz Instituto Oswaldo Cruz Departamento de Ultra-estrutura e Biologia CelularRio de Janeiro RJ ,Brasil

关键词: Toxoplasma gondii;    amylopectin granules;    tissue cysts;    ultrastructural cytochemistry;   

DOI  :  10.1590/S0074-02762003000700010

来源: SciELO

【 摘 要 】

Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. This technique can be used for study of T. gondii-host cell intracellular cycle, the differentiation tachyzoite-bradyzoite, and also for the formation of cysts into the host cells.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License