A Multiplex-PCR approach to identification of the Brazilian intermediate hosts of Schistosoma mansoni

Memórias do Instituto Oswaldo Cruz

Thda Vidigal²  Kg Magalhães¹  Jc Kissinger¹  Rl Caldeira¹  Ajg Simpson¹  Os Carvalho¹ 

[1] ,UFMG Instituto de Ciências Biológicas Departamento de ZoologiaBelo Horizonte MG ,Brasil

关键词: Biomphalaria;    Multiplex-PCR;    internal transcribed spacer 2;   

DOI  :  10.1590/S0074-02762002000900019

来源: SciELO

【 摘 要 】

Due to difficulties concerning morphological identification of planorbid snails of the genus Biomphalaria, and given a high variation of characters and in the organs with muscular tissue, we designed specific polymerase chain reaction (PCR) primers for Brazilian snail hosts of Schistosoma mansoni from available sequences of internal transcribed spacer 2 (ITS2) of the ribosomal RNA gene. From the previous sequencing of the ITS2 region, one primer was designed to anchor in the 5.8S conserved region and three other species-specific primers in the 28S region, flanking the ITS2 region. These four primers were simultaneously used in the same reaction (Multiplex-PCR), under high stringency conditions. Amplification of the ITS2 region of Biomphalaria snails produced distinct profiles (between 280 and 350 bp) for B. glabrata, B. tenagophila and B. straminea. The present study demonstrates that Multiplex-PCR of ITS2-DNAr showed to be a promising auxiliary tool for the morphological identification of Biomphalaria snails, the intermediate hosts of S. mansoni.

【 授权许可】

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