IgG Anti-IgE Autoantibodies in Visceral Leishmaniasis

Memórias do Instituto Oswaldo Cruz

Am Atta²  Mlb Sousa-atta²  A D'oliveira¹  Rp Almeida¹  Mi Araújo¹  Em Carvalho¹ 

[1] ,Universidade Federal da Bahia Faculdade de Farmácia Departamento de Análises Clínicas e ToxicológicasSalvador BA ,Brasil

关键词: IgE;    IgG anti-IgE;    protein G;    visceral leishmaniasis;    schistosomiasis mansoni;   

DOI  :  10.1590/S0074-02762002000100017

来源: SciELO

【 摘 要 】

Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa epsilon-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera.

【 授权许可】

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