Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species

Memórias do Instituto Oswaldo Cruz

Ear Rosa¹  Rt Rosa¹  Cv Pereira¹  Mfg Boriollo¹  Jf Höfling¹ 

[1] ,Unicamp Faculdade de Odontologia de Piracicaba Laboratório de Microbiologia e ImunologiaPiracicaba SP ,Brasil

关键词: polyacrylamide gel electrophoresis;    multilocus enzyme electrophoresis;    Candida;    numerical analysis;   

DOI  :  10.1590/S0074-02762000000600009

来源: SciELO

【 摘 要 】

Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Candida species commonly isolated from oral cavity of humans by numerical taxonomy methods. The obtained data revealed that sodium dodecyl sulfate polyacrylamide gel electrophoresis is more efficient in grouping strains in their respective species while MLEE has much limited resolution in organizing all strains in their respective species-specific clusters. MLEE technique must be regarded for surveys in which just one species of Candida is involved.

【 授权许可】

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