Genetic Data Showing Evolutionary Links between Leishmania and Endotrypanum

Memórias do Instituto Oswaldo Cruz

Elisa Cupolillo²  Luiza Or Pereira¹  Octávio Fernandes¹  Marcos P Catanho¹  Júlio C Pereira¹  Enrique Medina-acosta¹  Gabriel Grimaldi Jr¹ 

[1] ,Departamento de Imunologia

关键词: Leishmania colombiensis;    Leishmania equatorensis;    Endotrypanum;    multilocus enzyme electrophoresis;    molecular characterization;    numerical analysis;    sialidase activity;    kDNA;   

DOI  :  10.1590/S0074-02761998000500020

来源: SciELO

【 摘 要 】

Striking similarities at the morphological, molecular and biological levels exist between many trypanosomatids isolated from sylvatic insects and/or vertebrate reservoir hosts that make the identification of medically important parasites demanding. Some molecular data have pointed to the relationship between some Leishmania species and Endotrypanum, which has an important epidemiological significance and can be helpful to understand the evolution of those parasites. In this study, we have demonstrated a close genetic relationship between Endotrypanum and two new leishmanial species, L. (V.) colombiensis and L. (V.) equatorensis. We have used (a) numerical zymotaxonomy and (b) the variability of the internal transcribed spacers of the rRNA genes to examine relationships in this group. The evolutionary trees obtained revealed high genetic similarity between L. (V.) colombiensis, L. (V.) equatorensis and Endotrypanum, forming a tight cluster of parasites. Based on further results of (c) minicircle kDNA heterogeneity analysis and (d) measurement of the sialidase activity these parasites were also grouped together.

【 授权许可】

CC BY   
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