A GBP 130 derived peptide from Plasmodium falciparum binds to human erythrocytes and inhibits merozoite invasion in vitro

Memórias do Instituto Oswaldo Cruz

Jorge E Suarez¹  Mauricio Urquiza¹  Hernando Curtidor¹  Luis E Rodriguez¹  Marisol Ocampo¹  Elizabeth Torres¹  Fanny Guzman¹  Manuel Elkin Patarroyo¹ 

[1] ,Universidad Nacional de Colombia Hospital San Juan de Dios Instituto de InmunologíaBogotá,Colombia

关键词: Plasmodium falciparum;    GBP 130;    binding assay;   

DOI  :  10.1590/S0074-02762000000400009

来源: SciELO

【 摘 要 】

The malarial GBP 130 protein binds weakly to intact human erythrocytes; the binding sites seem to be located in the repeat region and this region's antibodies block the merozoite invasion. A peptide from this region (residues from 701 to 720) which binds to human erythrocytes was identified. This peptide named 2220 did not bind to sialic acid; the binding site on human erythrocyte was affected by treatment with trypsin but not by chymotrypsin. The peptide was able to inhibit Plasmodium falciparum merozoite invasion of erythrocytes. The residues F701, K703, L705, T706, E713 (FYKILTNTDPNDEVERDNAD) were found to be critical for peptide binding to erythrocytes.

【 授权许可】

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