Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction

Memórias do Instituto Oswaldo Cruz

Marize Pereira Miagostovich¹  Flávia Barreto Dos Santos¹  Eliane Saraiva M De Araújo¹  Juarez Dias¹  Hermann G Schatzmayr¹  Rita Maria Ribeiro Nogueira¹ 

关键词: dengue;    diagnosis;    virological surveillance;    polymerase chain reaction;   

DOI  :  10.1590/S0074-02761997000500006

来源: SciELO

【 摘 要 】

A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecting and typing dengue viruses in suspected cases, allowing a rapid identification of new serotypes in endemic areas

【 授权许可】

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