Dengue virus type 3 isolation from Aedes aegypti in the municipality of Nova Iguaçu, State of Rio de Janeiro

Memórias do Instituto Oswaldo Cruz

Ricardo Lourenço-de-oliveira²  Nildimar A Honório²  Márcia G Castro²  Hermann G Schatzmayr¹  Marize P Miagostovich¹  João Cr Alves²  Wellington C Silva¹  Paulo J Leite¹  Rita Mr Nogueira¹ 

[1] ,Fiocruz Instituto Oswaldo Cruz Laboratório de Transmissores de Hematozoários

关键词: dengue;    Aedes aegypti;    Aedes albopictus;    reverse transcription;    polymerase chain reaction;    virus isolation;    Rio de Janeiro;    Brazil;   

DOI  :  10.1590/S0074-02762002000600009

来源: SciELO

【 摘 要 】

In a prospective field study conducted from July 2000 to June 2001, adult Aedes aegypti and Ae. albopictus mosquitoes were caught from the municipality of Nova Iguaçu, State of Rio de Janeiro, Brazil. Virus isolation in Ae. albopictus clone C6/36 cell line and a semi-nested reverse transcription-polymerase chain reaction detected only dengue virus type 3 in three pools of Ae. aegypti, despite the co-circulation of DEN-1, DEN-2 and DEN-3 serotypes in that area. No viruses were detected in Ae. albopictus mosquitoes. This virological surveillance consists in a sentinel system alerting for dengue outbreaks.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License