Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library

Memórias do Instituto Oswaldo Cruz

Glória R. Franco²  Andrew J. G. Simpson¹  Sérgio D. J. Pena² 

[1] ,UFMG Instituto de Ciências Biológicas Departamento de BioquímicaBelo Horizonte,Brasil

关键词: Schistosoma mansoni;    automated DNA sequencing;    EST;    gene identification;   

DOI  :  10.1590/S0074-02761995000200015

来源: SciELO

【 摘 要 】

We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequencers. ESTs can be used to identify genese onf the basis of their homology whith sequences from other species deposited in DNA or protein databases. Trasncripts with sequences without matches in teh databases may represent novel parasite-specific genes. This approach has shown to be very efficient and in less than two years a broad range of novel genes has already been ascertained, more than doubling the number of known S. mansoni genes.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License