A new repetitive DNA sequence from Trypanosoma cruzi

Memórias do Instituto Oswaldo Cruz

Leila De Mendonça-lima¹  Yara M. Traub-cseko¹ 

[1] ,Instituto Oswaldo Cruz Departamento de Bioquímica e Biologia Molecular Rio de Janeiro,Brasil

关键词: Trypanosoma cruzi;    DNA sequence;    repetitive DNA;   

DOI  :  10.1590/S0074-02761991000400020

来源: SciELO

【 摘 要 】

Tandemly repeated DNA sequences are found in the genome of higher eukaryotes, and have also been demonstrated in Trypanosoma cruzi. Repeated DNA sequences are potentially useful for the diagnostic detection of T. cruzi (A. Gonzales et al., 1984, Proc. Natl. Acad. Sci. USA, 81: 3356-3360). We have isoleted two clones from a genomic library of T. cruzi (Y strain) that contain, in one clone a family of at least seven copies of a repetitive sequence of approximately 600 base pairs, and in the other an independent copy of the same sequence. One copy of the repetition (HSP) and the independent clone (HCR) were sequenced by the Sanger procedure (Fig.). This sequence hybridized to four strains of T. cruzi tested and did not hybridize to eleven species of trypanosotids from five different Genera, being a good candidate for diagnostic assays. GenBank accession numbers: HSP#m31919, HCR#31920.

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