Molecular characterization of clinical multiresistant isolates of Acinetobacter sp. from hospitals in Porto Alegre, State of Rio Grande do Sul, Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Alessandra Einsfeld Ferreira²  Desirée Padilha Marchetti¹  Gabriela Rosa Da Cunha¹  Lyvia Moreira De Oliveira¹  Daiane Bopp Fuentefria²  Aline Gehlen Dall Bello¹  Afonso Luis Barth¹  Gertrudes Corção² 

[1] ,Universidade Federal do Rio Grande do SulPorto Alegre RS

关键词: Acinetobacter sp. blaOXA-23;    Clonal dissemination;    ERIC-PCR;    Acinetobacter sp. blaOXA-23;    Disseminação clonal;    ERIC-PCR;   

DOI  :  10.1590/S0037-86822011000600014

来源: SciELO

【 摘 要 】

INTRODUCTION: Hospitals around the world have presented multiresistant Acinetobacter sp. outbreaks. The spread of these isolates that harbor an increasing variety of resistance genes makes the treatment of these infections and their control within the hospital environment more difficult. This study aimed to evaluate the occurrence and dissemination of Acinetobacter sp. multiresistant isolates and to identify acquired resistance genes. METHODS: We analyzed 274 clinical isolates of Acinetobacter sp. from five hospitals in Porto Alegre, RS, Brazil. We evaluated the susceptibility to antimicrobial, acquired resistance genes from Ambler's classes B and D, and performed molecular typing of the isolates using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) technique. RESULTS: A high (68%) percentage of multiresistant isolates of Acinetobacter sp. was observed, and 69% were resistant to carbapenems. We identified 84% of isolates belonging to species A. baumannii because they presented the gene blaOXA-51. The gene blaOXA-23 was detected in 62% of the isolates, and among these, 98% were resistant to carbapenems. Using the ERIC-PCR technique, we identified clones of Acinetobacter sp. spread among the four hospitals analyzed during the sampling period. CONCLUSIONS: The data indicate the dissemination of Acinetobacter sp. isolates among hospitals and their permanence in the hospital after one year.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License