Nosocomial infection and characterization of extended-spectrum β-lactamases-producing Enterobacteriaceae in Northeast Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Afonso Gomes Abreu¹  Sirlei Garcia Marques²  Valério Monteiro-neto²  Roberto Morais Luz De Carvalho¹  Azizedite Guedes Gonçalves¹ 

[1] ,Universidade Federal do Maranhão Programa de Pós-Graduação em Ciências da Saúde São Luís MA

关键词: Enterobacteriaceae;    Antimicrobial resistance;    ESBLs;    Nosocomial infection;    Enterobactérias;    Resistência antimicrobiana;    ESBLs;    Infecção hospitalar;   

DOI  :  10.1590/S0037-86822011000400008

来源: SciELO

【 摘 要 】

INTRODUCTION: Extended spectrum β-lactamases (ESBLs) are enzymes that degrade β-lactam antibiotics and have been reported to be an important cause of nosocomial infection in worldwide. METHODS: During 2009, 659 enterobacteria strains were isolated from different clinical specimens and tested for ESBL production. The disk approximation test, combined disk method and addition of clavulanic acid were used for phenotypic detection of the ESBL-producing strains and PCR for detection of the blaTEM and blaCTX-M genes. RESULTS: Among the isolates, 125 were ESBL producers. The blaCTX-M and blaTEM genes were detected in 90.4% and 75% of the strains, respectively. Most strains were isolated from urine. Klebsiella pneumoniae was the most prevalent organism. Microorganisms presented high resistance to the antibiotics. CONCLUSIONS: These results support the need for extending ESBL detection methods to different pathogens of the Enterobacteriaceae family because these methods are only currently standardized by the CLSI for Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca and Proteus mirabilis. Carbapenems were the antibiotic class of choice for the treatment of infections caused by ESBL-producing Enterobacteriaceae.

【 授权许可】

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