期刊论文详细信息
Revista da Sociedade Brasileira de Medicina Tropical
Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease
Ivna De Melo Magalhães2  Rebeca Vasquez Novo Martins2  Renata Oliveira Vianna1  Solange Artimos Oliveira1  Silvia Maria Baeta Cavalcanti2 
[1] ,Universidade Federal Fluminense Instituto Biomédico Departamento de Microbiologia e ParasitologiaNiterói RJ
关键词: Human herpesvirus 6;    Exanthem subitum;    Multiplex PCR;    Indirect immunofluorescence assay;    Primary infection;    Herpesvírus humano tipo 6;    Exantema súbito;    Multiplex PCR;    Imunofluorescência indireta;    Infecção primária;   
DOI  :  10.1590/S0037-86822011005000021
来源: SciELO
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【 摘 要 】

INTRODUCTION: Exanthem subitum is a classical rash disease of early childhood caused by human herpesvirus 6B (HHV-6B). However, the rash is frequently misdiagnosed as that of either measles or rubella. METHODS: In this study, a nested multiplex polymerase chain reaction (PCR) was used to diagnose HHV-6B primary infection, differentiate it from infections caused by HHV-6A and compare it to antibody avidity tests. The samples were separated into case group and control group according to the results of the indirect immunofluorescence assay (IFA) technique. RESULTS: From the saliva samples analyzed, HHV-6A DNA was detected in 3.2% of the case group and in 2.6% of the control group. Regarding HHV-6B, PCR detected viral DNA in 4.8% of the case group and in 1.3% of the control group. Among the serum samples studied, a frequency of 1.7% was determined for HHV-6A in the case group and 1.2% in the control group. PCR did not detect HHV-6B DNA in serum samples. The sensitivity and specificity of the PCR technique ranged from 0% to 4.8% and 97.5% to 100%, respectively, compared to IFA. CONCLUSIONS: The PCR technique was not suitable for diagnosing primary infection by HHV-6B in children with exanthematic disease and should not substitute the IFA.

【 授权许可】

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