Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease

Revista da Sociedade Brasileira de Medicina Tropical

Oliveira, Solange Artimos¹  Cavalcanti, Silvia Maria Baeta¹  Martins, Rebeca Vasquez Novo¹  Universidade Federal Fluminense, Niterói¹  Vianna, Renata Oliveira¹  Magalhães, Ivna de Melo¹ 

关键词: Human herpesvirus 6;    Exanthem subitum;    Multiplex PCR;    Indirect immunofluorescence assay;    Primary infection;   

DOI  :  10.1590/S0037-86822011005000021

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: Exanthem subitum is a classical rash disease of early childhood caused by human herpesvirus 6B (HHV-6B). However, the rash is frequently misdiagnosed as that of either measles or rubella.METHODS: In this study, a nested multiplex polymerase chain reaction (PCR) was used to diagnose HHV-6B primary infection, differentiate it from infections caused by HHV-6A and compare it to antibody avidity tests. The samples were separated into case group and control group according to the results of the indirect immunofluorescence assay (IFA) technique.RESULTS: From the saliva samples analyzed, HHV-6A DNA was detected in 3.2% of the case group and in 2.6% of the control group. Regarding HHV-6B, PCR detected viral DNA in 4.8% of the case group and in 1.3% of the control group. Among the serum samples studied, a frequency of 1.7% was determined for HHV-6A in the case group and 1.2% in the control group. PCR did not detect HHV-6B DNA in serum samples. The sensitivity and specificity of the PCR technique ranged from 0% to 4.8% and 97.5% to 100%, respectively, compared to IFA.CONCLUSIONS: The PCR technique was not suitable for diagnosing primary infection by HHV-6B in children with exanthematic disease and should not substitute the IFA.

【 授权许可】

Unknown