Antimicrobial resistance and investigation of the molecular epidemiology of Listeria monocytogenes in dairy products

Revista da Sociedade Brasileira de Medicina Tropical

Fernanda De Nes²  Gustavo Pelicioli Riboldi¹  Ana Paula Guedes Frazzon¹  Pedro Alves D'azevedo¹  Jeverson Frazzon² 

[1] ,Federal University of Rio Grande do Sul Program in Food Science and Technology Porto Alegre RS ,Brazil

关键词: Listeria monocytogenes;    PCR-REA;    RAPD;    Dairy products;    Listeria monocytogenes;    PCR-REA;    RAPD;    Produtos lácteos;   

DOI  :  10.1590/S0037-86822010000400009

来源: SciELO

【 摘 要 】

INTRODUCTION: Listeria monocytogenes is a ubiquitous microorganism in nature and is responsible for listeriosis, an infectious disease caused by consumption of contaminated food. METHODS: Molecular characterization was performed on 19 strains of Listeria monocytogenes (serovars 1/2a, 1/2b, 4b and 4c), isolated from dairy products in Rio Grande do Sul, Brazil. The molecular techniques applied were random amplification of polymorphic DNA and restriction enzyme analysis. In addition to the molecular analysis, the antimicrobial resistance profile was determined. RESULTS: The strains studied showed a low degree of diversity. In relation to the antimicrobial resistance profile of those microorganisms from the samples analyzed, all of them were susceptible to the antimicrobials tested. CONCLUSIONS: The molecular techniques that were used presented good discriminatory power for the strains studied. Furthermore, all of the samples that were analyzed were susceptible to the antimicrobials tested.

【 授权许可】

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