Prevalence of Listeria species in camel sausages from retail markets in Aydin province in Turkey and RAPD analysis of Listeria monocytogenes isolates

Irish Veterinary Journal

Filiz Kok²  Hasan Basri Ertas³  Gokben Ozbey¹ 

[1] Vocational School of Health Services, University of Firat, 23119 Elazig/Turkey;Department of Food Science and Technology, Faculty of Veterinary Science, University of Adnan Menderes, 09016 Isikli, Aydin/Turkey;Department of Microbiology, Faculty of Veterinary Science, University of Firat, 23119 Elazig/Turkey

关键词: RAPD;    PCR;    Listeria monocytogenes;    Camel sausage;   

Others  :  1150462
DOI  :  10.1186/2046-0481-59-6-342

【 摘 要 】

Samples were taken from 100 camel sausages from the different retail markets in Aydin province in the south-west of Turkey and they were tested for the presence of Listeria spp by biochemical methods. Samples were enriched using Listeria Enrichment Broth and they were inoculated onto Listeria Selective Agar. Listeria monocytogenes was isolated from nine samples (9%), Listeria innocua from 14 samples (14%) and Listeria welshimeri from two samples(2%). A 701 bp fragment of listeriolysin O sequence for L. monocytogenes was amplified using specific primers by polymerase chain reaction (PCR) for confirmation of the identification. A random primer (OPA-11) was used in a random amplified polymorphic DNA (RAPD) assay. This detected five different band profiles amongst the L. monocytogenes isolates, indicating a relatively large amount of genetic heterogeneity amongst the nine isolates. The study has highlighted the need for improved strategies for food safety, in particular appropriate hygienic precautions to avoid contamination of sausage during the manufacturing process and appropriate preservation techniques during storage and transport, to prevent transmission of Listeria spp to consumers at home and abroad.

   

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Autio T, Hielm S, Miettinen M, Sjoberg AM, Aarnisalo K, Bjorkroth J, Mattila-Sandholm T, Korkeala H: Sources of Listeria monocytogenes contamination in a coldsmoked rainbow trout processing plant detected by pulsed-field gel electrophoresis typing. Applied and Environmental Microbiology 1999, 65:150-155.
• [2]Baloga AO, Harlander SK: Comparison of methods for discrimination between strains of Listeria monocytogenes from epidemiological surveys. Applied and Environmental Microbiology 1991, 57:2324-2331.
• [3]Bibb WF, Gelin BG, Weaver R, Schwartz B, Plikaytis BD, Reeves MW, Pinner RW, Claire V, Broome CV: Analysis of clinical and food-borne isolates of Listeria monocytogenes in the United States by multilocus enzyme electrophoresis and application of the method to epidemiological investigations. Applied and Environmental Microbiology 1990, 6:2133-2141.
• [4]Border PM, Howard JJ, Plastow GS, Siggens KW: Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction. Letters in Applied Microbiology 1990, 11:158-162.
• [5]Breer C, Schopfer K: Listerien in Nahrungsmitteln. Schweizerische Medizinische Wochenschrift 1989, 119:306-311.
• [6]Breuer VJ, Prandl O: Nachweis von Listerien und deren vorkommen in hackfleisch und mettwürsten österreich. Archiv für Lebensmittelhygiene 1988, 39:28-30.
• [7]Byun SK, Jung SC, Yoo HS: Random amplification of polymorphic DNA typing of Listeria monocytogenes isolated from meat. International Journal of Food Microbiology 2001, 69:227-235.
• [8]Ciftcioglu G: Investigations on presence of Listeria monocytogenes in minced meat, sausage and meats. In PhD Thesis. University of Istanbul, Istanbul, Turkey; 1992.
• [9]Farber JM, Sanders GW, Johnston MA: A survey of various foods for the presence of Listeria species. Journal of Food Protection 1989, 52:456-458.
• [10]Franciosa G, Pourshaban M, Gianfranceschi M, Aureli P: Genetic typing of human and food isolates of Listeria monocytogenes from episodes of listeriosis. European Journal of Epidemiology 1998, 14:205-210.
• [11]Guven A, Patir B: Studies of the Listeria species in some meat and meat products consumed in Elazig city. Turkish Journal of Veterinary and Animal Sciences 1998, 22:205-212.
• [12]Incze K: Dry fermented sausages. Meat Science 49 Supplement 1998, 1:169-177.
• [13]Johnson JL, Doyle MP, Cassens RG: Listeria monocytogenes and Listeria spp in meat and meat products. A review Journal of Food Protection 1990, 53:81-91.
• [14]Paziak-Domanska B, Boguslawska E, Wieckowska-Szakiel M, Kotlowski R, R ózalska B, Chmiela M, Kur J, Dabrowski W, Rudnicka W: Evaluation of the API test, phosphatidylinositol-specific phospholipase C activity and PCR method in identification of Listeria monocytogenes in meat foods. FEMS Microbiology Letters 1999, 171:209-214.
• [15]Schmidt U, Seeliger HPR, Glenn E, Langer B, Leistner L: Listerienfunde in rohen fleischerzeugnissen. Fleischwirtschaft 1988, 68:1313-1316.
• [16]Seeliger HPR, Jones D: Genus Listeria. In Bergey's Manual of Systematic Bacteriology. Second edition. Edited by Kandler O, Weiss N. Baltimore: Williams and Wilkins; 1986:1235-1245.
• [17]Uyttendaele M, De Troy P, Debevere J: Incidence of Listeria monocytogenes in different types of meat products on the Belgian retail market. International Journal of Food Microbiology 1999, 53:75-80.
• [18]Wesley IV, Ashton F: Restriction enzyme analysis of Listeria monocytogenes strains associated with food-borne epidemics. Applied and Environmental Microbiology 1991, 57:969-975.