Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate

Revista da Sociedade Brasileira de Medicina Tropical

Ana Paula Ferreira²  Thadeu Côrrea²  Rosângela Cunha¹  Marcos José Marques²  Maria Angela Montesano²  Maria Aparecida Souza²  Henrique Couto Teixeira² 

[1],Universidade Federal de Juiz de Fora Instituto de Ciências Biológicas Deptartamento de Parasitologia, Microbiologia e ImunologiaJuiz de Fora MG

关键词: Paracoccidioidomycosis;    Antigens;    Metaperiodate;    Diagnosis;    Paracoccidiodomicose;    Antígenos;    Metaperiodato;    Diagnóstico;   

DOI  :  10.1590/S0037-86822008000400001

来源: SciELO

【 摘 要 】

In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis.

【 授权许可】

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