Assessment of chemiluminescence and PCR effectiveness in relation to conventional serological tests for the diagnosis of Chagas' disease

Revista da Sociedade Brasileira de Medicina Tropical

Aulísia Maria Vieira Duarte²  Hélida Monteiro De Andrade¹  Semíramis Jamil Hadad Do Monte¹  Vicente De Paulo Coelho Peixoto De Toledo²  Tânia Mara Pinto Dabés Guimarães² 

[1] ,University of Minas Gerais Faculty of Pharmacy Department of Clinical and Toxicological AnalysisBelo Horizonte MG

关键词: Chagas' disease;    Diagnosis;    Serology;    Chemiluminescence;    PCR;    Doença de Chagas;    Diagnóstico;    Sorologia;    Quimioluminescência;    PCR;   

DOI  :  10.1590/S0037-86822006000400014

来源: SciELO

【 摘 要 】

While testing 414 sera for the diagnosis of Chagas' disease, the conventional reactions of indirect hemagglutination, indirect immunofluorescence and the immunosorbent assay showed a sensitivity of 95.7%, 100% and 98.2% and a specificity of 98%, 98% and 96.4%, respectively, and an excellent association using Fisher's exact test. Chemiluminescence presented 100% sensitivity and 89.6% specificity, while PCR showed 100% specificity and 1.2% sensitivity. It is believed that the three conventional serological reactions are still adequate for diagnosing Chagas' disease.

【 授权许可】

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