Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection

Revista da Sociedade Brasileira de Medicina Tropical

Pedro Carnieli Junior²  Armando Moraes Ventura¹  Edison Luiz Durigon² 

[1] ,Institute Pasteur São PauloSão Paulo SP

关键词: Rabies;    N Gene;    Reverse transcription polymerase chain reaction;    Probe;    Hybridization;    Raiva;    Gene N;    Transcrição reversa-reação em cadeia da polimerase;    Sonda;    Hibridação;   

DOI  :  10.1590/S0037-86822006000200005

来源: SciELO

【 摘 要 】

A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100% agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this technique even more and confers specificity to the diagnosis. The technique described in this work will be useful in rabies diagnosis laboratories, once the cost is compatible with traditional rabies diagnostic techniques.

【 授权许可】

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