Diversity of Trypanosoma cruzi stocks and clones derived from Chagas disease patients: I-Behavioral characterization in vitro

Revista da Sociedade Brasileira de Medicina Tropical

L. Lauria-pires¹  J.m. Santana¹  F.s. Tavares¹  A.r.l. Teixeira¹ 

[1] ,Universidade de BrasíliaBrasília DF

关键词: Trypanosoma cruzi;    Behavioral characterization;    Genetic diversity;    Trypanosoma cruzi;    Caracterização do comportamento;    Diversidade genética;   

DOI  :  10.1590/S0037-86821997000300003

来源: SciELO

【 摘 要 】

In this study, we isolated Trypanosoma cruzi from chronic Chagas heart disease and from megaesophagus patients. The parasite stock hSLU239 (heart disease) yielded clones h1 and h2, whereas stock mSLU142 (megaesophagus) yielded clones m1, m2, m3 and m4. The parasite growth kinetics, doubling time and differentiation in axenic liquid medium showed broad behavioral diversity. It was shown that a particular pattern of behavior for a parental stock could not necessarily be assigned for subsequent clones. This study indicates that i) each Chagas disease patient is infected with several T. cruzi populations; ii) clonal lines derived from patient samples may have different biological characteristics from the original isolate; and that iii) additional behavioral and/or molecular markers are required for further characterization of Trypanosoma cruzi stocks and clones derived from Chagas disease patients in order to identify correlations with pathology.

【 授权许可】

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