QUANTITATIVE VS. CONVENTIONAL PCR FOR DETECTION OF HUMAN ADENOVIRUSES IN WATER AND SEDIMENT SAMPLES

Revista do Instituto de Medicina Tropical de São Paulo

Rodrigo Staggemeier¹  Marina Bortoluzzi¹  Tatiana Moraes Da Silva Heck¹  Fernando Rosado Spilki¹  Sabrina Esteves De Matos Almeida¹ 

关键词: Adenovirus;    Quantitative PCR;    Conventional PCR;    Water;    Sediment;   

DOI  :  10.1590/S0036-46652015000400005

来源: SciELO

【 摘 要 】

SUMMARY Human Adenoviruses (HAdV) are notably resistant in the environment. These agents may serve as effective indicators of fecal contamination, and may act as causative agents of a number of different diseases in human beings. Conventional polymerase chain reaction (PCR) and, more recently, quantitative PCR (qPCR) are widely used for detection of viral agents in environmental matrices. In the present study PCR and SYBR(r)Green qPCR assays were compared for detection of HAdV in water (55) and sediments (20) samples of spring and artesian wells, ponds and streams, collected from dairy farms. By the quantitative methodology HAdV were detected in 87.3% of the water samples and 80% of the sediments, while by the conventional PCR 47.3% and 35% were detected in water samples and sediments, respectively.

【 授权许可】

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