Principles and applications of polymerase chain reaction in medical diagnostic fields: a review

Brazilian Journal of Microbiology

Marcela Agne Alves Valones¹  Rafael Lima Guimarães¹  Lucas André Cavalcanti Brandão¹  Paulo Roberto Eleutério De Souza¹  Alessandra De Albuquerque Tavares Carvalho¹  Sergio Crovela¹ 

[1] ,Universidade Federal de PernambucoRecife PE ,Brasil

关键词: Conventional PCR;    Real-Time PCR;    Molecular Biology;    Methods of molecular detection;    PCR convencional;    PCR em Tempo Real;    Biologia molecular;    métodos de detecção molecular;   

DOI  :  10.1590/S1517-83822009000100001

来源: SciELO

【 摘 要 】

Recent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry. Among these methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements. PCR is an excellent technique for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real-Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical diagnostics and research laboratories. Due to its capacity to generate both qualitative and quantitative results, Real-Time PCR is considered a fast and accurate platform. The aim of the present literature review is to explore the clinical usefulness and potential of both conventional PCR and Real-Time PCR assays in diverse medical fields, addressing its main uses and advances.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License