Rickettsia parkeri: a Rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern Uruguay

Revista do Instituto de Medicina Tropical de São Paulo

José M. Venzal²  Agustín Estrada-peña¹  Aránzazu Portillo¹  Atilio J. Mangold¹  Oscar Castro²  Carlos G. De Souza²  María L. Félix²  Laura Pérez-martínez¹  Sonia Santibánez¹  José A. Oteo¹ 

[1] ,Universidad de la República Facultad de Veterinaria Departamento de Parasitología VeterinariaSalto,Uruguay

关键词: Rhipicephalus sanguineus;    Amblyomma triste;    Rickettsia parkeri;    Spotted fever group (SFG);    Uruguay;   

DOI  :  10.1590/S0036-46652012000300003

来源: SciELO

【 摘 要 】

At first Rickettsia conorii was implicated as the causative agent of spotted fever in Uruguay diagnosed by serological assays. Later Rickettsia parkeri was detected in human-biting Amblyomma triste ticks using molecular tests. The natural vector of R. conorii, Rhipicephalus sanguineus, has not been studied for the presence of rickettsial organisms in Uruguay. To address this question, 180 R. sanguineus from dogs and 245 A. triste from vegetation (flagging) collected in three endemic localities were screened for spotted fever group (SFG) rickettsiosis in southern Uruguay. Tick extracted DNA pools were subjected to PCR using primers which amplify a fragment of the rickettsial gltA gene. Positive tick DNA pools with these primers were subjected to a second PCR round with primers targeting a fragment of the ompA gene, which is only present in SFG rickettsiae. No rickettsial DNA was detected in R. sanguineus. However, DNA pools of A. triste were found to be positive for a rickettsial organism in two of the three localities, with prevalences of 11.8% to 37.5% positive pools. DNA sequences generated from these PCR-positive ticks corresponded to R. parkeri. These findings, joint with the aggressiveness shown by A. triste towards humans, support previous data on the involvement of A. triste as vector of human infections caused by R. parkeri in Uruguay.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License