The sialotranscriptome of Amblyomma triste, Amblyomma parvum and Amblyomma cajennense ticks, uncovered by 454-based RNA-seq

Particle and Fibre Toxicology

Sandra Regina Maruyama¹  Isabel Kinney Ferreira de Miranda-Santos¹  Matias Pablo Juan Szabó²  Maria Marlene Martins²  Cláudio Mafra⁵  Higo Nasser Santanna Moreira⁵  Beatriz Rossetti Ferreira⁴  Elen Anatriello⁴  José Marcos Ribeiro³  Luiz Gustavo Gardinassi¹  Gustavo Rocha Garcia¹ 

[1] Department of Biochemistry and Immunology, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil;School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, MG, Brazil;Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA;Department of Maternal and Child and Public Health Nursing, Ribeirão Preto School of Nursing, University of São Paulo, Ribeirão Preto, SP, Brazil;Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, MG, Brazil

关键词: RNA-seq;    Transcriptome;    Saliva;    Salivary gland;    Amblyomma triste;    Amblyomma parvum;    Amblyomma cajennense;    Ticks;   

Others  :  1150394
DOI  :  10.1186/1756-3305-7-430

 received in 2014-06-11, accepted in 2014-08-22,  发布年份 2014

【 摘 要 】

Background

Tick salivary constituents antagonize inflammatory, immune and hemostatic host responses, favoring tick blood feeding and the establishment of tick-borne pathogens in hosts during hematophagy. Amblyomma triste, A. cajennense and A. parvum ticks are very important in veterinary and human health because they are vectors of the etiological agents for several diseases. Insights into the tick salivary components involved in blood feeding are essential to understanding vector-pathogen-host interactions, and transcriptional profiling of salivary glands is a powerful tool to do so. Here, we functionally annotated the sialotranscriptomes of these three Amblyomma species, which allowed comparisons between these and other hematophagous arthropod species.

Methods

mRNA from the salivary glands of A. triste, A. cajennense and A. parvum ticks fed on different host species were pyrosequenced on a 454-Roche platform to generate four A. triste (nymphs fed on guinea pigs and females fed on dogs) libraries, one A. cajennense (females fed on rabbits) library and one was A. parvum (females fed on dogs) library. Bioinformatic analyses used in-house programs with a customized pipeline employing standard assembly and alignment algorithms, protein databases and protein servers.

Results

Each library yielded an average of 100,000 reads, which were assembled to obtain contigs of coding sequences (CDSs). The sialotranscriptome analyses of A. triste, A. cajennense and A. parvum ticks produced 11,240, 4,604 and 3,796 CDSs, respectively. These CDSs were classified into over 100 distinct protein families with a wide range of putative functions involved in physiological and blood feeding processes and were catalogued in annotated, hyperlinked spreadsheets. We highlighted the putative transcripts encoding saliva components with critical roles during parasitism, such as anticoagulants, immunosuppressants and anti-inflammatory molecules. The salivary content underwent changes in the abundance and repertoire of many transcripts, which depended on the tick and host species.

Conclusions

The annotated sialotranscriptomes described herein richly expand the biological knowledge of these three Amblyomma species. These comprehensive databases will be useful for the characterization of salivary proteins and can be applied to control ticks and tick-borne diseases.

【 授权许可】

   
2014 Garcia et al.; licensee BioMed Central Ltd.

【 图 表 】

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