Identification of respiratory virus in infants with congenital heart disease by comparison of different methods

Revista do Instituto de Medicina Tropical de São Paulo

Tatiana Mitiko Kanashiro²  Lucy Santos Vilas Boas²  Ana Maria Thomaz¹  Tania Regina Tozetto-mendoza²  Mônica Setsuko¹  Clarisse Martins Machado² 

[1] ,Universidade de São Paulo Instituto de Medicina Tropical de São Paulo Laboratório de VirologiaSão Paulo SP ,Brazil

关键词: Respiratory viruses;    Congenital heart disease;    Direct immunofluorescence assay;    Multiplex PCR;    PCR;   

DOI  :  10.1590/S0036-46652011000500001

来源: SciELO

【 摘 要 】

Respiratory virus infections are the main cause of infant hospitalization and are potentially severe in children with congenital heart disease (CHD). Rapid and sensitive diagnosis is very important to early introduction of antiviral treatment and implementation of precautions to control transmission, reducing the risk of nosocomial infections. In the present study we compare different techniques in the diagnosis of respiratory viruses in CHD infants. Thirty-nine samples of nasopharyngeal aspirate were obtained from CHD infants with symptoms of respiratory infection. The Multiplex PCR (Seeplex® RV 12 ACE Detection) driven to the detection of 12 respiratory viruses was compared with the direct immunofluorescence assay (DFA) and PCR, both targeting seven respiratory viruses. The positivity found by DFA, Multiplex and PCR was 33.3%, 51.3% and 48.7%, respectively. Kappa index comparing DFA and Multiplex, DFA and PCR and PCR and Multiplex PCR was 0.542, 0.483 and 0.539, respectively. The concordance between techniques was considered moderate. Both Multiplex PCR (p = 0.001) and PCR (p = 0.002) detected significantly more respiratory virus than DFA. As the performance of the tests may vary, the combination of two or more techniques may increase diagnostic sensitivity favoring the diagnosis of co-infections, early introduction of antiviral therapy and implementation of appropriate measures.

【 授权许可】

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