Excretion-secretion products and proteases from live Sporothrix schenckii yeast phase: immunological detection and cleavage of human IgG

Revista do Instituto de Medicina Tropical de São Paulo

Daniel Da Rosa²  Elbio Gezuele²  Luis Calegari²  Fernando Goñi¹ 

[1] ,Universidad de la República Facultad de Medicina Departamento de Parasitología y MicologíaMontevideo,Uruguay

关键词: Sporothrix schenckii;    Excretion-secretion product;    Human immunoglobulin;    Proteases;    Yeast phase;   

DOI  :  10.1590/S0036-46652009000100001

来源: SciELO

【 摘 要 】

Antigenic preparations from Sporothrix schenckii usually involve materials from mixed cultures of yeast and mycelia presenting cross-reactions with other deep mycoses. We have standardized pure yeast phase with high viability of the cells suitable to obtain specific excretion-secretion products without somatic contaminations. These excretion-secretion products were highly immunogenic and did not produce noticeable cross-reactions in either double immunodiffusion or Western blot. The antigenic preparation consists mainly of proteins with molecular weights between 40 and 70 kDa, some of them with proteolytic activity in mild acidic conditions. We also observed cathepsin-like activity at two days of culture and chymotrypsin-like activity at four days of culture consistent with the change in concentration of different secreted proteins. The proteases were able to cleave different subclasses of human IgG suggesting a sequential production of antigens and molecules that could interact and interfere with the immune response of the host.

【 授权许可】

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