Fecal specimens preparation methods for PCR diagnosis of human taeniosis

Revista do Instituto de Medicina Tropical de São Paulo

Cáris Maroni Nunes¹  Luiz Gustavo Ferraz Lima¹  Camila Santos Manoel¹  Rodrigo Norberto Pereira¹  Mauro Massaharu Nakano¹  José Fernando Garcia¹ 

[1] ,UNESP FOA Departamento de Apoio, Produção e Saúde AnimalAraçatuba SP ,Brasil

关键词: Taenia saginata;    DNA extraction;    Polymerase chain reaction (PCR);    Fecal specimens;   

DOI  :  10.1590/S0036-46652006000100010

来源: SciELO

【 摘 要 】

Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol® reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit®. Best DNA extraction results were achieved after eggs disruption with glass beads, either with phenol/chloroform/isoamilic alcohol, DNAzol® reagent or QIAmp DNA stool mini kit®.

【 授权许可】

CC BY-NC   
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