Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction

Revista do Instituto de Medicina Tropical de São Paulo

Liana Konovaloff Jannotti-passos¹  Cecilia Pereira De Souza¹ 

[1] ,FIOCRUZ Centro de Pesquisas René Rachou Laboratório de MalacologiaBelo Horizonte MG ,Brasil

关键词: Schistosoma mansoni;    Biomphalaria tenagophila;    Biomphalaria straminea;    Susceptibility;    LS-PCR technique;   

DOI  :  10.1590/S0036-46652000000500010

来源: SciELO

【 摘 要 】

In order to determine Schistosoma mansoni infection rates in Biomphalaria tenagophila and B. straminea, low stringency polymerase chain reaction (LS-PCR) technique was used as a complementary method to light exposure technique. LS-PCR has already been standardized in our laboratory to detect the trematode DNA in B. glabrata. Higher S. mansoni infection rates were detected using conventional method and LS-PCR. The parasite DNA profile was detected in both species after 7-day exposure to miracidia, using LS-PCR. This technique enables early detection of schistosomiasis transmission focuses, in endemic areas, before the beginning of cercariae shedding.

【 授权许可】

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