Production of TNF-a by primary cultures of human keratinocytes challenged with Loxosceles gaucho venom

Revista do Instituto de Medicina Tropical de São Paulo

Ceila M.s. MÁlaque¹  Maria Ori²  Sânia A. Santos¹  Dahir R. Andrade¹ 

[1] ,Universidade de São PauloSão Paulo SP ,Brasil

关键词: Loxosceles;    Keratinocytes;    Cell culture;    TNF-a;   

DOI  :  10.1590/S0036-46651999000300009

来源: SciELO

【 摘 要 】

Primary cultures of human keratinocytes were challenged with increasing doses from 10 ng/mL to 2 mg/mL of Loxosceles gaucho venom, responsible for dermonecrotic lesion in humans. TNF-a was investigated by bioassay and ELISA in the supernatant of the cultures challenged with 100 ng/mL, 500 ng/mL, 1 and 2 mg/mL of venom. TNF-a was detected by bioassay in the supernatant of cultures challenged with 100 ng/mL, after 6 h. The cytokine was detected by ELISA in the supernatant of the cells challenged with doses of l mg/mL, after 6 and 12 h. The results point out the capacity of this venom to activate the keratinocytes in primary cultures to produce TNF-a. The production of cytokines could contribute to the local inflammatory process in patients bitten by Loxosceles sp.

【 授权许可】

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