Entamoeba histolytica: antigenic characterization of axenic strains from Brazil

Revista do Instituto de Medicina Tropical de São Paulo

Semíramis Guimarães²  Haidée Urdaneta¹  Edward F. Silva²  Carlos Alberto P. Tavares¹ 

[1] ,Federal Minas Gerais University Institute of Biological Science Department of ParasitologyBelo Horizonte MG ,Brazil

关键词: Entamoeba histolytica;    Axenic strains;    Antigens;   

DOI  :  10.1590/S0036-46651991000100002

来源: SciELO

【 摘 要 】

Trophozoites from cultures of Entamoeba histolytica strains isolated and grown axenically in Brazil (ICB-CSP, ICB-462 and ICB-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain isolated and kept under axenic conditions in USA (HK-9). Hyperimmune sera, presenting high antibody titers with homologous and heterologous antigens, were obtained. The four strains in study revealed similar and complex electrophoretic and glycoproteic profiles showing polypep-tides with molecular weights ranging from 200 to less than 29 kDa. No significant differences were detected between the pathogenic and non-pathogenic strains.

【 授权许可】

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