Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains

Revista do Instituto de Medicina Tropical de São Paulo

Semíramis GuimarÃes²  Maria Inês T. Leme Sogayar²  Marcello Franco¹ 

[1] ,Universidade Estadual Paulista Instituto de Biociências Departamento de ParasitologiaBotucatu São Paulo ,Brasil

关键词: Giardia duodenalis;    Axenic strains;    Trophozoites;    Proteins;    Glycoproteins;    Antigens;   

DOI  :  10.1590/S0036-46652002000500001

来源: SciELO

【 摘 要 】

In the present study, we have analyzed by sodium docecyl sulphate - polyacrilamide gel electrophoresis (SDS-PAGE), immunoblotting and Concanavalin A blotting (Con A blotting) proteins of membrane fractions and soluble fractions obtained from Giardia duodenalis trophozoites of two axenic strains isolated in Brazil from a symptomatic (BTU-11) and an asymptomatic patient (BTU-10), as compared to the reference strain Portland 1. Both Brazilian strains showed a complex and homogeneous electrophoretic pattern of proteins, but some differences could be observed. Several glycoproteins were detected, particularly the proteins of 81, 72, 59 kDa and the protein of 62 kDa in the membrane proteins and cytosol, respectively. Many antigenic components were revealed by anti-Giardia rabbit IgG antibodies in the immunoblotting analysis. Among these components, the membrane protein of 32 kDa and the cytosol protein of 30 kDa could be related to giardin, as previously demonstrated.

【 授权许可】

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