Expressions of matrix metalloproteinases-1 and -9 and opioid growth factor in rabbit cornea after lamellar keratectomy and treatment with 1% nalbuphine

Arquivos Brasileiros de Oftalmologia

Miguel Ladino Silva¹  Alexandre Pinto Ribeiro¹  Germana Alegro Silva¹  Irma Ximena Barbosa Sanchez¹  Roberta Renzo¹  Ricardo Uscategui¹  Tiago Barbalho Lima¹  Marcela Aldrovani¹  José Luiz Laus¹ 

关键词: Cornea;    Photorefractive keratectomy;    Nalbuphine/therapeutic use;    Matrix metalloproteinases;    Receptors;    opioid;    Immunohistochemistry;    Animals;    Rabbits;    Córnea;    Ceratectomia fotorrefrativa;    Nalbufina/uso terapêutico;    Metaloproteinases da matriz;    Receptores opióides;    Imuno-histoquímica;    Animais;    Coelhos;   

DOI  :  10.5935/0004-2749.20150037

来源: SciELO

【 摘 要 】

ABSTRACT Purposes: To evaluate the effects of nalbuphine 1% on the expression of metalloproteinase 1 (MMP-1), metalloproteinase 9 (MMP-9), and opioid growth factor (OGF) in rabbit corneas after lamellar keratectomy. Methods: The rabbits were assigned to two groups: group nalbuphine (GN, n=30), which received 30 µL of nalbuphine 1% in 4 daily applications at regular intervals until corneal epithelialization, and group control (GC, n=30), which received physiological saline solution under the same conditions adopted in GN. The corneas were collected for immunohistochemistry on days 1, 3, 5, 7, and 9 after lamellar keratectomy, and the expressions of MMP-1, MMP-9, and OGF were analyzed. Results: The expressions of MMP-1 and MMP-9 increased until day 5 of the evaluation, with no differences observed between GN and GC (p>0.05). On days 7 and 9, significant reductions were observed in the expression of MMP-1 (p<0.01), with no differences observed between GN and GC (p>0.05). The expression of OGF was constant in all periods (p>0.05), restricted to the corneal epithelium, and there was no difference between the groups (p>0.05). Conclusions: The study results showed that nalbuphine 1% did not alter the expression patterns of MMP-1, MMP-9, and OGF in rabbit corneas after lamellar keratectomy.

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