Sublethal effect of concentrations of Metarhizium anisopliae (metsch.) sorok on the larval stage and immunologic characteristics of Diatraea flavipennella (box) (lepidoptera: crambidae)

Anais da Academia Brasileira de Ciências

Jennifer GuimarÃes¹  Edmilson J. Marques¹  ValÉria Wanderley-teixeira¹  Auristela C. De Albuquerque¹  Eliana M. Dos Passos¹  CÍnthia C.m. Silva¹  Álvaro A.c. Teixeira¹ 

关键词: Entomopathogen;    hemocytes;    histochemistry;    nitric oxide;    phenoloxidases;    sugarcane borer;    entomopatógeno;    hemócitos;    histoquí-mica;    óxido nítrico;    fenoloxidase;    Broca da cana-de-açúcar;   

DOI  :  10.1590/0001-3765201420130339

来源: SciELO

【 摘 要 】

This work aimed to evaluate the effects of Metarhizium anisopliae on Diatraea flavipennella and investigate their immune response. Was observed the effect of M. anisopliae against larvae of D. flavipennella sprayed at concentrations of 103, 104, 105 conidia / mL, in which showed differences relative the larval period, extending up to 72.0 days in treatment and 25.0 days in the control. The results for hemocytes revealed that the most frequent cells when sprayed at the concentrations of 103, 105, 107 conidia / mL were the prohemocytes, spherulocytes, plasmatocytes and granulocytes in relation to adipohemocytes and oenocytoids. The level of nitric oxide was different between the control and the concentration 107 spores / mL (24), while the activity of phenoloxidase was similar among treatments in 24 and higher concentration 107 spores / mL (60h). In biochemical profile of hemocytes was a change in carbohydrates, lipids and proteins in response to the fungus. The results indicate that the fungus M. anisopliae can be used in the Integrated Management of D. flavipennella by presenting pathogenicity and interfere with their development even when exposed to small concentrations.

【 授权许可】

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