DNA damage in children and adolescents with cardiovascular disease risk factors

Anais da Academia Brasileira de Ciências

Mariele Kliemann²  Daniel Prá¹  Luiza L. Müller¹  Liziane Hermes¹  Jorge A. Horta¹  Miriam B. Reckziegel¹  Miria S. Burgos¹  Sharbel W. Maluf¹  Silvia I.r. Franke¹  Juliana Da Silva² 

[1] ,Universidade Luterana do Brasil Programa de Pós-Gradução em Biologia Celular e Molecular Aplicada à Saúde Canoas RS ,Brasil

关键词: cardiovascular diseases;    Micronucleus test;    Comet assay;    diet;    anthropometry;    child;    adolescent;    doenças cardiovasculares;    Teste de micronúcleos;    Ensaio cometa;    dieta;    antropometria;    criança;    adolescente;   

DOI  :  10.1590/S0001-37652012005000039

来源: SciELO

【 摘 要 】

The risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low risk subjects (P<0.05). High risk subjects also showed a significant increase in DNA damage, which was higher than that provided by low and moderate risk subjects according to SCGE, but not according to the CBMN assay. Vitamin C intake was inversely correlated with DNA damage by SCGE, and micronucleus (MN) was inversely correlated with folate intake. The present results indicate an increase in DNA damage that may be a consequence of oxidative stress in young individuals with risk factors for CVD, indicating that the DNA damage level can aid in evaluating the risk of CVD.

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