Differentially displayed expressed sequence tags in Melipona scutellaris (Hymenoptera, Apidae, Meliponini) development

Anais da Academia Brasileira de Ciências

Flávia A. Santana²  Francis M.f. Nunes¹  Carlos U. Vieira²  Maria Alice M.s. Machado²  Warwick E. Kerr²  Wilson A. Silva Jr¹  Ana Maria Bonetti² 

[1] ,Universidade Federal de Uberlândia Instituto de Genética e Bioquímica Laboratório de GenéticaUberlândia MG ,Brasil

关键词: Melipona scutellaris;    gene expression;    DDRT-PCR;    Melipona scutellaris;    expressão gênica;    DDRT-PCR;   

DOI  :  10.1590/S0001-37652006000100008

来源: SciELO

【 摘 要 】

We have compared gene expression, using the Differential Display Reverse Transcriptase - Polymerase Chain Reaction (DDRT-PCR) technique, by means of mRNA profile in Melipona scutellaris during ontogenetic postembryonic development, in adult worker, and in both Natural and Juvenile Hormone III-induced adult queen. Six, out of the nine ESTs described here, presented differentially expressed in the phases L1 or L2, or even in both of them, suggesting that key mechanisms to the development of Melipona scutellaris are regulated in these stages. The combination HT11G-AP05 revealed in L1 and L2 a product which matches to thioredoxin reductase protein domain in the Clostridium sporogenes, an important protein during cellular oxidoreduction processes. This study represents the first molecular evidence of differential gene expression profiles toward a description of the genetic developmental traits in the genus Melipona.

【 授权许可】

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