Structure and anticoagulant properties of sulfated glycosaminoglycans from primitive Chordates

Anais da Academia Brasileira de Ciências

Mauro S. G. PavÃo¹ 

[1] ,Universidade Federal do Rio de Janeiro Hospital Universitário Clementino Fraga Núcleo de Glicobiologia Aplicada

关键词: ascidian;    heparin;    dermatan sulfate;    heparin cofactor II;    anticoagulant;    antithrombotic;    ascídias;    heparina;    dermatam sulfato;    heparina cofator II;    anticoagulante;    antitrombótico;   

DOI  :  10.1590/S0001-37652002000100007

来源: SciELO

【 摘 要 】

Dermatan sulfates and heparin, similar to the mammalian glycosaminoglycans, but with differences in the degree and position of sulfation were previously isolated from the body of the ascidian Styela plicata and Ascidia nigra. These differences produce profound effects on their anticoagulant properties. S. plicata dermatan sulfate composed by 2-O-sulfatedalpha-L-iduronic acid and 4-O-sulfated N-acetyl-beta-D-galactosamine residues is a potent anticoagulant due to a high heparin cofactor II activity. Surprisingly, it has a lower potency to prevent thrombus formation on an experimental model and a lower bleeding effect in rats than the mammalian dermatan sulfate. In contrast, A. nigra dermatan sulfate, also enriched in 2-O-sulfated alpha-L-iduronic acid, but in this case sulfated at O-6 of the N-acetyl-beta-D-galactosamine units, has no in vitro or in vivo anticoagulant activity, does not prevent thrombus formation but shows a bleeding effect similar to the mammalian glycosaminoglycan. Ascidian heparin, composed by 2-O-sulfated alpha-L-iduronic acid, N- and 6-O-sulfated glucosamine (75%) and alpha-L-iduronic acid, N- and 6-O-sulfated glucosamine (25%) disaccharide units has an anticoagulant activity 10 times lower than the mammalian heparin, is about 20 times less potent in the inhibition of thrombin by antithrombin, but has the same heparin cofactor II activity as mammalian heparin.

【 授权许可】

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